This course focuses on computational quantification of biologically relevant micro/macroscopic structures in biomedical images. Students will learn how (i) raw data is acquired before digitization; (ii) to read, display, and interpret various medical image data types using a computer; (iii) to detect, segment, and quantify heterogeneous structures in biomedical images; (iv) to leverage features extracted from biomedical images for classification; (v) to setup experiments in MATLAB via script writing for biomedical image analysis. At the beginning of the course, an overview of the medical imaging will be given. Motivation for conducting quantitative analysis of medical images will be discussed. Forward measurement models describing the microscopic and macroscopic imaging system output will be reviewed.