The field of Structural Biology sits at the interface of numerous disciplines, including biochemistry, biology, and physics and promotes the use of a myriad of technologies to investigate the structure-function relationships of biological macromolecules at the molecular level. Understanding the interplay between structure and function provides insight into numerous fundamental biological processes that underlie healthy and diseased states. Generating sufficient quantities of purified, stable, and active protein lies at the heart of all structure-function studies. This course is designed to provide students with an understanding of the principles behind protein production. The first part of the class focuses on methods utilized to generate suitable protein samples, including heterologous expression in bacterial and eukaryotic systems, column chromatographic separations, and stability analyses. The second part of class provides an overview of methods and technologies used to determine and analyze protein structure at the molecular level, including the nature of enzyme catalysis, X-ray crystallography, cryo-electron microscopy, nuclear magnetic resonance, and other solution-based methods.